subtom_rotx_motl

Transforms a given MOTL file so that it matches a tomogram rotated or not rotated by IMOD’s ‘clip rotx’ command.

This MOTL manipulation script uses one MATLAB compiled scripts below:

Options

Directories

tomogram_dir

Absolute path to the folder where the tomograms used in the INPUT motive list are stored.

scratch_dir

Absolute path to the folder with the input to be processed. Other paths are relative to this one.

mcr_cache_dir

Absolute path to MCR directory for the processing.

exec_dir

Directory for executables

Variables

rotx_motl_exec

Rotx motive list executable

File Options

input_motl_fn

Relative path and name of the input MOTL file to be transformed.

output_motl_fn

Relative path and name of the output MOTL file.

Rotx Options

tomo_row

Row number of allmotl for tomogram numbers.

do_rotx

If the following is set to 1 the input MOTL will be transformed in the same way as done by ‘clip rotx’. If it is set to 0 the input MOTL will be transformed by the inverse operation (a positive 90 degree rotation about the X-axis).

Example

tomogram_dir="/net/dstore2/teraraid/dmorado/subTOM_tutorial/data/tomos/bin4"

scratch_dir="${PWD}"

mcr_cache_dir="${scratch_dir}/mcr"

exec_dir="/net/dstore2/teraraid/dmorado/software/subTOM/bin"

rotx_motl_exec="${exec_dir}/MOTL/subtom_rotx_motl"

input_motl_fn="../bin4/combinedmotl/allmotl_1.em"

output_motl_fn="combinedmotl/allmotl_1.em"

tomo_row="7"

do_rotx="0"